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Home My WebLink AboutItem 10 PPP 09-02-2020 Operations Committee - Sewage Surveillance Project Update Wastewater Surveillance for --�-- COVID =19 Ik Presented by Lan Wiborg '- Director of Environmental Services Operations Committee September 2, 2020 MEMO a- -" o�N�y S A N I rq T�O� 10 newe. 9 :.. � c -o ti M-'— M D THE i _ 1 f. Keeping up with the COV113 =19 Headlines l� In a Los Angeles Times interview early last week,omwas quoted as saying, "I wouldn't go in the water if you paid me Of coronavirus $1 million right now."She posited that SC1entists are unsure SARS-CoV-2, the virus that causes COVID-19, effects at the beach could enter the ocean —through raw or poorly 9 days later, in a treated sewage — and then get kicked gy ROSANNA XIA Q follow-up article into the air alon the surf back APR 2, 2020 1 OT00 AM g zone. UPDATED OT18 AM a leading atmosphenc chemist But new research published after the interview offiffim has changed her thinking. The research at the Scripps Institution of oceanography,rfer, ell out her window at every Includes an accelerated ublication of a stud wants to y spots along the San in the journal Nature, which found that the runner, and biker she virus did not appear to remain infectious in Diego coast.I wouldnt fecal matter. ' g o in the water if you paid me $1 ht now, she said. "The main exposure risk to the water million rig The beach,in her estimation, is one of the recreation community remains sewage laces to be these days, as pollution and urban runoff into the ocean, most dangerous p which can increase after major storms such ., - - 1- -` - 1- - -1 .I--- 2 LI Zs Wastewater Surveillance The proof is in the sewage: hundreds of Yosemite visitors may have had coronavirus wdim „o What's poop got to do with it? O Midi 0 Nlw'a n Valley Call Use New Policy For Gars Use 09 M.I—Day What to Read Neat Yosemite national park sofficials nsuspect that hundredsYof.eisitors this summer ! ----' BlloNomber9La OE—roirnren.e.Er tly Report O p Sewage"Could aBecome Next VirusSurveillance Method, : EPA Says Gy it ® r Wastewater and SARS =CoV=2 Questions from Media/Public/Employees Is OCSD testing its sewage for SARS-CoV-2? Why? I When will OCSD receive preliminary results? I What can OCSD do with these results? Sewage surveillance for COVID-19 in OC? I Key Considerations sons from prior wastewater surveillance efforts I • Obtain expert advice i Establish current and future use cases I i Sustainable field and laboratory resources � Criteria for collaboration I i Research Partners 1111111111L . �� State Water Board Trussell 0 • Add-on to APR-2 project TECHNOLOGIES INC LIF0A MIA CA • Multi-benefit for water reuse agencies Water Boards THE Water • No existing method STATF, WATER RFSOURCES CONTROL BOARD Research RF,GIONAL WATFR QUALITY CONTROL BOARDS FOUNDATION University of Arizona THE UNIVERSITY • Extensive experience with SARS-CoV-1 OF ARIZONA • Existing method for wastewater matrix Stanford University UNIVERSITY OF MICHIGAN • Innovation rant for method development g p Stanford yCCWRP SOUTHERN CALIF0RNIA • Nationwide recruitment of WWTPs RESEAR L WATER University RESEARCH PROJECT 6 State Water Board : �V L -c DPR Research Projects t r , "D P R-2" �.� Pathogen Monitoring Lk Quantitative Microbial Assessment Outbreak Monitoring Risk v - Non-Targeted Analysis Control of I !�+ � Source Control Chemical Peaks and low Molecular - Weight Compounds 7 Image Source:B.Pecson/Trussell Technologies,Inc. State Water Board : DPR=2 Help to develop Direct Potable Reuse Criteria Method • Samples from five major CA POTWs • Sample collection since November 2019 Development • Added SARS-CoV-2 to study in April 2020 • Method optimization • Comparability assessment Crypto (cyst/L) Giardia (oocyst/L) Enterovirus culture Detectionand (MPN/L) Adenovirus culture • (MPN/L) Enterovirus molecular (GC/L) Adenovirus molecular (GC/L) Norovirus GIA P R Treatment molecular (GC/L) Norovirus GIB Effectiveness molecular (GC/L) Norovirus GII molecular (GC/L) 8 1+i DPR=2 Preliminary Data 1.E+09 1-E-03 1,E+08 U 0 � C 1.E+07 1 E 04 c NI 0 N2 C 1.E 06 — Confirmed U1.E+OS • Infections i 1 E n5 E ZI,1+04 • E N 1.E+03 U U O 1 E-06 U 1.E•02 Q �n 1.E+01 8R 2 Z are RNA 11+00 1 f % genes from 4/1 4/15 4/29 5/13 6/10 6/24 SARS-CoV-2 Undergoing QA IQC Review — Do Not Gte 9 Image Source:B.Pecson/Trussell Technologies,Inc. %I�K.-0�2 k University of Arizona r' i 1 The University of Arizona says it caught a dorm's Detection covid-19 outbreak before it • started. Its secret weapon: Quantification Poop. Graduate students and employees process nasal swabs from coronavirus tests in a lab at the University of Arizona in Tucson on Aug.24.(Cheney Orr/Bloomberg News) By Jaclyn Pelser TreatmentWW August 28.2020 at 5:50 AM EDT Effectiveness 10 ' c University of Arizona OCSD Plant 1 COVID-19 Monitoring (Weekly thru 7/7/2020) 80000 -- N1 Marker 0 , — a) 4 N2 Marker }, L 60000 C: a) N1 Method Detection Limit a) 0- C v N2 Method Detection Limit 0 U 0':- 40000 N u O E V 0 � C 20000 < tkD ---------------'---------- � p ------� ------{------------- ; ------f----------------------}------- ----- ------ }------ , ---- ,LO ,ti0 ,ti0 ,LO ,LO ,LO ,y0 ,LO ,LO ,1 ,LO ,LO e`0 ,LO ,LO s(I 11 Image Source:University of Arizona/OCSD �Comparing Results :� ri A THE UNIVERSITY oa ® OF ARIZONA Water Boards OCSD Plant 1 COVID-19 Monitoring J 1.E+09 1.E-03 (Weekly thru 7/7/2020) 11+08 _ 80000 c -- =N1Marker 2 1,E+07 1,E•04 ` NI 0 L YI v N2 Marker 6000o c 1.E+06 N1 v a N1 Method D tection Limit , —Confirmed :,E+04 E+05 Infections o v N2 Method D tection Limit U • l.E 05 N o 40000 2 ■ v 0 I • ■ E yo N 1.E�03 N 20000 o r In - L---- r U 1.1-02 1.E06 J� 0 ------ ------Tj........ ----------- � 1.E+01 0,�0 Oti0 010 Oti0 0 Oti0 Oti0 Oti0 Oti0 0�0 Oti0 Oti Oti0 Oti0 1.E+00 1.E•07 h h h 0 0 0 1 4/1 4/15 4/29 5/13 5/27 6/10 6/24 12 Image Source:University of Arizona/OCSD Image Source:B.Pecson/Trussell Technologies,Inc. %I�K.-0�2 k r' Stanford University Method Development .a.,.: oai`��...,.,n e6rea^Vla,na •, aoeam aro�..p '. •� • -M UNITED • STATES cnr a�01d mn a,n.gn wen o n Detection and Quantification i l a o in ve°� w� o- Samples from US WWTPs Data 25 CA WWTPs Management 24 WWTPs outside CA • Archived 1,700 samples to date • Analyzed 2 POTWs to date Data Modeling Image Source:A.Boehm/Stanford University 13 %I�K.-0�2 k 1 Stanford University Method Development • SARS-CoV-2 affinity for solids • gPCR vs. digital droplet PCR RT-QPCR 15000 digital droplet RT-PCR 1,000 •R ix • ix o pox o pox • sox 10000 Z Q i0000 1-4 ; Z i'_• 'M �000 5000 Q1 Im Z � T l N a M a U-) a a+ a o a Q U-) a N a M a W-) a M a o a a u, a N 3 N M N 3 N M 7 V D N 7 N 7 N 7 N z ` z N M 1fJ O N N M to Qf Ln N N N M 4 fV N fV fV �_ M M M M M M M M M Q Image Source:A.Boehm/Stanford University 14 %I�K.-0�2 k 1 Stanford University Preliminary Results 250 CIO • Primary settled solids data 0�200 — 7�smoothed new cases -Fa i-- LL? = 150 .S U • Tracking with confirmed clinical cases 00 100 E.E • Data analysis and modeling in progress 0 50 o Lot ov��A 6000 Cannot Use to Predict Prevalence 5000 a4000 • • Missing key variable — virus shed in feces N 3000 Z 2000 • Prediction of COVID-19 unrealistically high '°°° 0 . « 6000 —3 sample smoothed N t and N2 5000 Recommendations 4000 3000 • PH information must guide sampling Z 2000 • Supply chain issues '°°0 _ _, 1 M� 29 Mar 26 Apr 24 May 21 Jun Image Source:A.Boehm/Stanford University 15 4C WN.W,-.A, CDC Perspective L_ %6 i � • Sewage is an efficient pooled sample of community (or sub-community) infection prevalence • Captures sub-clinical infections • Independent of healthcare-seeking behavior and testing access • Data available within days of shedding onset versus up to 2-week lag for other data • Data collection at CDC to facilitate national disease surveillance interpretation and public health actions Information and mage Source:A.Kirby/CDC 16 y PIN National Wastewater Surveillance System 71161 Health Departments Utilities Laboratories + + ono coo F,3 000c onnno \ o 000c c000c — + 131313 coo 0000CDC COVID Data Tracker if '� ExpWe and understand coronavirusdiseaw(COVID-19) I ►�,,,`,0 AP av��.H SfiRVIC�S.G — — w Worid Heahh ---:� — •rh1 �fq[PROtE�M1\ Organization Image Source:A.Kirby/CDC 17 CDC 's Role in NWSS ■ Ensure data comparability across J jurisdictions 11 1■ ■ Analyze data to provide public National Notifiable Diseases Surveillance System Data Flow health interpretation and guidance Case Reporting Case Notification CDC Nospigls Local/Regional/State NNDSS/DHIS CDC Programs Public Health Authority ......�'� ��;il) ■ Summarize and make national data �� ,1eaoata °� �'' available for states and public a°` '� t� securer taeo0 ��. program Healthcare / no � �~ Malysis ■ agency Providers . ' Pabh(HeaahhEventsSupport inter-health o(Intematioml \ HU �I Diu (on(em communication forpublic health ins ration Engine a O z World Health � g � , � ■ labOratOtiK and Other Tools d ; Organization o : action MOR Publication M SecmeTransport ® J Based on state reportable conditions Based on nationally notifiable conditions Image Source:A.Kirby/CDC 18 1� Next Steps Continue Coordination with OCHCA Sewer Dynamics Sampling and Analytical Methods glum, g _ ell" Technical OCSD Regional Advisors Advisors Ian Pepper,PhD James Herberg, PE Director,WEST Center General Manager University of Arizona Haruna(Nana)Feldman, PhD,MPH Group Program Manager Ron Coss,MSPH,MPA,BCES Data&Performance Analytics Unit Chief Administrative Office Charles Gerba, PhD Laboratory,Monitoring,and Compliance County of San Diego Manager Professor,Environmental Science University of Arizona Sam Choi,PhD 20 Environmental Supervisor Walter Betancourt,PhD Assoc.Research Professor,Env.Science University of Arizona Roya Sohanaki, PE Alissa Dratch,MPH Engineering Manager Epidemiologist Communicable Disease Control Greg Kester,PE Orange County Health Care Agency Director of Renewable Resources Programs Jennifer Cabral,MPA CA Assoc.of Sanitation Agencies Administrative Services Manager �� 41 j� r 21